XTP3TPA / DCTPP1 Protein (His Tag)

13.05.2013

Sino Biological Inc. liefert XTP3TPA / DCTPP1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über XTP3TPA / DCTPP1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/DCTPP1-XTP3TPA-Protein-Antibody-a-5925.html

Produkt-Information von XTP3TPA / DCTPP1 Protein

Synonym	XTP3TPA, DCTPP1
Protein Construction	A DNA sequence encoding the mature form of human XTP3TPA (NP_077001.1) (Met1-Thr170) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von XTP3TPA / DCTPP1 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: DCTPP1 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human XTP3TPA consists of 185 amino acids and predicts a molecular mass of 20.5 KDa. It migrates as an approximately 21 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM Tris, 10% Glycerol, 100mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von XTP3TPA / DCTPP1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von XTP3TPA / DCTPP1 Protein

DCTPP1 hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates. It has a strong preference for modified dCTP. DCTPP1’s activity is highest with 5-iodo-dCTP, followed by 5-bromo-dCTP, unmodified dCTP, 5-methyl-dCTP and 5-chloro-dCTP. DCTPP1 also hydrolyzes 2-chloro-dATP and 2-hydroxy-dATP with lower efficiency, and has even lower activity with unmodified dATP, dTTP and dUTP (in vitro). DCTPP1 does not hydrolyze ATP, UTP, ITP, GTP, dADP, dCDP or dGTP. It may protect DNA or RNA against the incorporation of non-canonical nucleotide triphosphates. DCTPP1 may also protect cells against inappropriate methylation of CpG islands by DNA methyltransferases.

References

Stelzl U. et al., 2005, Cell. 122 (6): 957-68.
Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903.
Moroz OV. et al., 2005, J Mol Biol. 347 (2): 243-55.