PPC-DC / PPCDC Protein (His Tag)

13.05.2013

Sino Biological Inc. liefert PPC-DC / PPCDC Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über PPC-DC / PPCDC Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/PPC-DC-PPCDC-Protein-Antibody-a-5923.html

Produkt-Information von PPC-DC / PPCDC Protein

Synonym	PPCDC, COAC
Protein Construction	A DNA sequence encoding the mature form of human PPCDC (AAH14409.1) (Met1-Ser204) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von PPC-DC / PPCDC Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: PPCDC protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human PPCDC consists of 219 amino acids and predicts a molecular mass of 24.3 KDa. It migrates as an approximately 24 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, 15% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von PPC-DC / PPCDC Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PPC-DC / PPCDC Protein

PPC-DC, also known as PPCDC, belongs to the HFCD (homo oligomeric flavin containing Cys decarboxylase) superfamily which takes a part in the biosynthesis of coenzyme A (CoA) from pantothenate (Vitamin B). Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. This process include several steps: the phosphorylation of pantothenate, the conversion of 4’-hosphopantothenate to 4''-phosphopantetheine, the adenylation by phosphopantetheine adenylyltransferase to form dephospho-CoA and the phosphorylation by dephospho-CoA kinase to form CoA. PPC-DC, one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine.

References

Zody MC. et al., 2006, Nature. 440: 671-5.
Ota T. et al., 2004, Nat Genet. 36: 40-5.
Clark HF. et al., 2003, Genome Res. 13: 2265-70.