PMKI / PMVK Protein (His Tag)

13.05.2013

Sino Biological Inc. liefert PMKI / PMVK Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über PMKI / PMVK Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/PMVK-phosphomevalonate-kinase-Protein-Antibody-a-5928.html

Produkt-Information von PMKI / PMVK Protein

Synonym	PMVK, PMKI
Protein Construction	A DNA sequence encoding the mature form of human PMVK(Q15126) (Met1-Leu192) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von PMKI / PMVK Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: PMKI protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human PMVK consists of 207 amino acids and predicts a molecular mass of 23.8 KDa. It migrates as an approximately 24 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM MOPS, 150mM KCl, 1mM DTT, pH 7.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von PMKI / PMVK Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PMKI / PMVK Protein

PMVK is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of PMVK is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis.

References

Danielsen JM. et al., 2011, Mol Cell Proteomics. 10 (3): M110.003590.
Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
Ellinor PT. et al., 2012, Nat Genet. 44 (6): 670-5.