Mein Blog umfasst die aktuellesten News, Forschungsleistung, neuesten Produkte und Service von Sino Biological Inc. und die aktuelle News von Life Sciences. Sie können sich hier wissenschaftlich informieren.Willkommen zu meinem Blog.Viel Spaß!
| Immunogen | Recombinant Human VEGF / VEGFA / VEGF165 protein (Catalog#11066-HNAB) |
| Antibody Type | Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform ) |
| Clone ID | 105 |
| Ig Type | Rabbit IgG |
| Formulation | 0.2 μm filtered solution in PBS with 5% trehalose |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Human VEGF / VEGFA / VEGF165 (rh VEGF / VEGFA / VEGF165; Catalog#11066-HNAB; P15692-4; Met 1-Arg 191). |
| Specificity | Human VEGF / VEGFA / VEGF165 |
| Has cross-reactivity in ELISA with Mouse VEGF164 Human VEGF121 No cross-reactivity in ELISA with Human VEGFC
| |
| Western blot | This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human VEGF165 in WB. Using a DAB detection system, the detection limit for Human VEGF165 is approximately 0.5 ng/lane under non-reducing conditions and not recommended under reducing conditions. |
| Direct ELISA | This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human VEGF165. The detection limit for Human VEGF165 is approximately 0.00975 ng/well. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. As its name implies, VEGF-A activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (stimulation monocyte/macrophage, neurons, cancer cells, kidney epithelial cells). VEGF-A is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. Alternatively spliced transcript variants of VEGF-A, encoding either secreted or cell-associated isoforms, have been identified. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform and is a heparin-binding growth factor with mitogenic activity for vascular endothelial cells. Human VEGF165 shares 88%, 96%, 95% and 93% aa sequence identity with mouse, porcine, canine and feline VEGF165, respectively.
Sino Biological Inc. liefert VEGF / VEGFA / VEGF165 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.
Weitere Informationen über VEGF / VEGFA / VEGF165 Antikörper lesen Sie bitte: http:www.sinobiological.com/VEGF-VEGFA-VEGF165-Antibody-g-9217.html
/https%3A%2F%2Fassets.over-blog.com%2Ft%2Fcedistic%2Fcamera.png)