VE-Cadherin / CD144 / CDH5 Antikörper (Antigen Affinität reinigt)

15.03.2012

Produkt-Information von VE-Cadherin / CD144 / CDH5 Antikörper

Immunogen	Recombinant Mouse VE-Cadherin / CD144 / CDH5 protein (Catalog#50192-M08H)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Mouse VE-Cadherin / CD144 / CDH5 (rM VE-Cadherin / CD144 / CDH5; Catalog#50192-M08H; NP_033998.2; Met 1-Ala 592). VE-Cadherin / CD144 / CDH5 specific IgG was purified by Mouse VE-Cadherin / CD144 / CDH5 affinity chromatography.

VE-Cadherin / CD144 / CDH5 Antikörper Usage Guide

Specificity	Mouse VE-Cadherin / CD144 / CDH5
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CDH5 in WB. Using a DAB detection system, the detection limit for Mouse CDH5 is approximately 2 ng/lane under non-reducing conditions and reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CDH5. The detection limit for Mouse CDH5 is approximately 0.00975 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von VE-Cadherin / CD144 / CDH5 Antikörper

Cadherins (Calcium dependentadhesion molecules) are a class of transmembrane proteins. They play important roles incell adhesion, ensuring that cells within tissues are bound together. They are dependent oncalcium (Ca2+)ionsto function. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, is single-pass type I membrane protein. It is expressed in endothelial tissues and brain andis involved in loss of heterozygosity events in breast and prostate cancer. CDH5 is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, CDH5 may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.

References

Angst, B. et al., 2001, J Cell Sci. 114 (Pt 4): 629-41.
Duguay, D. et al., 2003, Dev. Biol.253: 309-23.
Bayas, MV. et al., 2005, Biophysical. Journal.90 (4): 1385.
Crosby, CV, et al., 2005,Blood. 105 (7): 2771-6.
Hulpiau, P. et al., 2009, Int. J. Biochem. Cell Biol.41 (2): 349-69.

Sino Biological Inc. liefert VE-Cadherin / CD144 / CDH5 Antikörper (Antigen Affinität reinigt) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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