VASN / Vasorin Protein (His Tag)

27.11.2012

Sino Biological Inc. liefert VASN / Vasorin Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über VASN / Vasorin Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/VASN-Vasorin-Protein-g-9914.html

Produkt-Information von VASN / Vasorin Protein

Synonym	VASN, SLITL2
Protein Construction	A DNA sequence encoding the human VASN (Q6EMK4) (Met1-Pro575) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von VASN / Vasorin Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: VASN protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Cys 24
Molecular Mass	The recombinant human VASN consists of 563 amino acids and predicts a molecular mass of 61.1 KDa. It migrates as an approximately 66-76 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von VASN / Vasorin Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von VASN / Vasorin Protein

Vasorin contains tandem arrays of a characteristic leucine-rich repeat motif, an epidermal growth factor-like motif, and a fibronectin type III-like motif at the extracellular domain. It is a type I membrane protein that is expressed predominantly in vascular Smooth muscle cells. Vasorin directly binds to TGF-beta and attenuates TGF-beta signaling in vitro. This suggests that down-regulation of vasorin expression contributes to neointimal formation after vascular injury and that vasorin modulates cellular responses to pathological stimuli in the vessel wall. It may act as an inhibitor of TGF-beta signaling. Vasorin interacts with TGFB1, TGFB2 and TGFB3.

References

Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.
Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
Lee KA. et al., 2011, J Biol Chem. 286 (48): 41530-8.