Tie2 / CD202b / TEK Protein (GST Tag)

11.06.2012

Produkt-Information von Tie2 / CD202b / TEK Protein

Synonym	TEK, TIE2, VMCM, VMCM1
Protein Construction	A DNA sequence encoding the human TEK(NP_000450)(Gln771-Ala1124)was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von Tie2 / CD202b / TEK Protein

Purity	> 92 % as determined by SDS-PAGE	SDS-PAGE: Tie2 / CD202b / TEK protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human TEK /GST chimera consists of 591 amino acids and has a calculated molecular mass of 68.3 kDa. The recombinant protein migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 8.0,10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von Tie2 / CD202b / TEK Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Verwandte Produkte & Themen von Tie2 / CD202b / TEK Protein

Related Areas:

Enzyme>>Protein Kinase>>Receptor Tyrosine Kinase>>Tie2/CD202b

Signal Transduction>>Protein Kinase>>Receptor Tyrosine Kinase>>Tie2/CD202b

Stem Cell>>Hematopoietic Stem Cell (HSC)>>Hemangioblast>>Hemangioblast Marker>>Tie2/CD202b

Stem Cell>>Hematopoietic Stem Cell (HSC)>>Hemangioblast>>Endothelial Cell Marker>>Tie2/CD202b

Cancer>>Growth Factor & Receptor>>Angiopoietin/Tie>>Tie2/CD202b

Cancer>>Growth Factor & Receptor>>Receptor Tyrosine Kinase>>Tie2/CD202b

Immunology>>Cluster of Differentiation>>Other>>Tie2/CD202b

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
Tie2	Human	Tie2/Fc Protein, Recombinant	10700-H03H
Tie2	Human	Tie2 Protein, Recombinant	10700-H08H
Tie2	Human	Tie2 / CD202b / TEK Protein, Recombinant, with GST Tag	10700-H20B1
Tie2	Mouse	Tie2 / TEK (aa 770-1122) Protein, Recombinant, with GST Tag	51087-M20B1
Tie2	Mouse	Tie2 / TEK Protein, Recombinant	51087-M08H
Tie2	Rat	Tie2 / CD202b / TEK Protein, Recombinant	80063-R02H

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human Tie2	WB, ELISA	Mouse Monoclonal Antibody	10700-MM01
Human Tie2	WB, ELISA	Rabbit Polyclonal Antibody	10700-RP01
Human Tie2	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	10700-RP02
Human Tie2	WB, ELISA	TEK / TIE2 / CD202b Antibody	10700-R002

Beschreibung von Tie2 / CD202b / TEK Protein

Tie2/Tek, together with Tie1/Tie, are members of the receptor tyrosine kinase (RTK) subfamily, and possess a unique extracellular domain containing two immunoglobulin-like loop domains flanking three epidermal growth factor (EGF) repeats followed by three fibronectin-type I II repeats, and a split tyrosine kinase domain in the cytoplasmic region. Tie2 is expressed predominantly on endothelial and hematopoietic progenitor cells and is essential for blood vessel formation and maintenance. The identified ligand angiopoietin-1 (Ang-1) binds Tie2 receptor with high-affinity and induces the signaling pathway of both cell migration and cell survival via the related mediator such as GRB2 and SH-PTP2. Accordingly, the Tie2 may trigger crucial endothelial responses during embryonic vascular development and during pathologic vascular growth, as well as the inflammatory responses with the key regulator ABIN-2. Recent study has revealed that Ang1-Tie2 signaling exerts different function at cell-cell contacts and cell-substratum contacts dependent upon the spatial localization of Tie2. Deficiency in this gene is associated with inherited venous malformations.

References

Partanen J. et al., 1992, Mol Cell Biol. 12: 1698-707.
Sato TN. et al., 1993, Proc. Natl. Acad. Sci. 90: 9355-8.
Jones N. et al., 1999, J. Biol. Chem. 274: 30896-905.
Fukuhara S. et al., 2008, Nat. Cell. Biol. 10: 513-26.
Marron MB. et al., 2007, J Biol Chem. 282: 30509-17.
Huqhes DP. et al., 2003, Circ Res. 92: 630-6.

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