PRKAG1 & PRKAB1 & PRKAA1 Heterotrimer Protein (GST Tag)

11.06.2012

Produkt-Information von PRKAG1 & PRKAB1 & PRKAA1 Heterotrimer Protein

Protein name	heterotrimer of protein kinase, AMP-activated, alpha 1 catalytic subunit, beta 1 non-catalytic subunit and gamma 1 non-catalytic subunit
Protein Construction	A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270) ,constructed the plasmid 2.; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von PRKAG1 & PRKAB1 & PRKAA1 Protein

Purity	> 94 % as determined by SDS-PAGE	SDS-PAGE: PRKAG1 & PRKAB1 & PRKAA1 Heterotrimer protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant heterotrimer of human PRKAG1 / PRKAB1 / PRKAA1 comprises 1397 (331+270+796) amino acids and has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass of the heterotrimer is approximately 38, 40 & 95 KDa respectively in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of 50mM Tris ,200mM NaCl, 2mM GSH,1mM EDTA ,1mM DTT, 10%gly, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von PRKAG1 & PRKAB1 & PRKAA1 Heterotrimer Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PRKAG1 & PRKAB1 & PRKAA1 Heterotrimer Protein

AMP-activated protein kinase (AMPK) is an important metabolic stress-sensing protein kinase induced by AMP. It can be detected in a number of tissues, including the liver, brain, and skeletal muscle. AMPK is a heterotrimer comprised by three proteins (α, β, and γ subunits) that together make a functional enzyme, conserved from yeast to humans. Seven known genes encode the subunit isoforms (α1, α2, β1, β2, γ1, γ2, γ3) and all are expressed in the heart. The most common isoforms expressed in most cells are the α1(PRKAA1), β1(PRKAB1), and γ1(PRKAG1) isoforms. AMPK induces a cascade of events within cells in response to the ever changing energy charge of the cell. Mammalian AMPK is a stable alphabetagamma heterotrimer comprising a catalytic alpha (PRKAA) and two non-catalytic subunits, beta (PRKAB) and gamma (PRKAG). AMPK activation results in the stimulation of hepatic fatty acid oxidation and ketogenesis, inhibition of cholesterol synthesis, lipogenesis, and triglyceride synthesis, inhibition of adipocyte lipolysis and lipogenesis, stimulation of skeletal muscle fatty acid oxidation and muscle glucose uptake, and modulation of insulin secretion by pancreatic beta-cells.

References

Adams J. et al., 2004, Protein Sci. 13 (1): 155-65.
Stapleton D. et al., 1996, J Biol Chem. 271 (2): 611-4.
Hallows KR. et al., 2009, Am J Physiol. 296 (4): C672-81.
Winder WW. et al., 1999, AmJPhysiol. 277 (1Pt1): E1-10.

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