Thyroid peroxidase / TPO Protein (His Tag)

25.04.2012

Produkt-Information von Thyroid peroxidase / TPO Protein

Synonym	MSA, TDH2A, TPX
Protein Construction	A DNA sequence encoding the human TPO (P07202-1) extracellular domain (Met 1- Arg846, 257 Ser/Ala, 725 Pro/Thr)) was fused with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von Thyroid peroxidase / TPO Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: Thyroid peroxidase / TPO protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Cys 15
Molecular Mass	The secreted recombinant human TPO comprises 842 amino acids and has a predicted molecular mass of 93.8 kDa. The apparent molecular mass of rh TPO (1-846) is approximately 90-100 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH7.4, 10%gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von Thyroid peroxidase / TPO Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Thyroid peroxidase / TPO Protein

Thyroid peroxidase, also known as TPO, is a membrane-bound glycoprotein which belongs to the peroxidase family, XPO subfamily. It acts as an enzyme and plays a central role in thyroid gland function. Thyroid peroxidase/TPO functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in thyroid peroxidase/TPO gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Thyroid peroxidase/TPO plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin to form precursors of the thyroid hormones T4 and T3. Defects in thyroid peroxidase/TPO are the cause of thyroid dyshormonogenesis 2A. A disorder due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.

References

McLachlan SM. et al., 2000, Int Rev Immunol. 19 (6): 587-618.
Nagasaka A. et al., 1976, J Clin Endocrinol Metab. 43 (1): 152-8.
Kimura S. et al., 1987, Proc Natl Acad. 84 (16): 5555-9.
Ruf J. et al., 2006, Arch Biochem Biophys. 445 (2): 269-77.

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