TCN2 / Transcobalamin-II Protein (His Tag)

15.05.2013

Sino Biological Inc. liefert TCN2 / Transcobalamin-II Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über TCN2 / Transcobalamin-II Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/TCN2-Protein-a-691.html

Produkt-Information von TCN2 / Transcobalamin-II Protein

Synonym	D22S676, D22S750, TC2,TCN2
Protein Construction	A DNA sequence encoding the human TCN2 (NP_000346.2) (Met 1-Trp 427) was expressed with a C-terminal polyhistidine tag.
Source	Human
Expression Host	CHO Stable Cell

QC Testing von TCN2 / Transcobalamin-II Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE TCN2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu 19
Molecular Mass	The secreted recombinant human TCN2 comprises 418 amino acids with a predicted molecular mass of 46.7 kDa. It migrates with the molecular weight of approximately 44 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von TCN2 / Transcobalamin-II Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von TCN2 / Transcobalamin-II Protein

Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl; vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 20 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities.

References

Rothenberg S P. et al., 1995, Baillieres Clin Haematol. 8 (3): 499-514.
Bibi H. et al., 1999, J Inherit Metab Dis. 22 (7): 765-772.
Seetharam B. et al., 2000, Vitam Horm. 59: 337-366.