Mouse IL5Ra / CD125 Protein (His Tag)

15.05.2013

Sino Biological Inc. liefert Mouse IL5Ra / CD125 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse IL5Ra / CD125 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/IL5RA-CD125-Protein-a-1442.html

Produkt-Information von IL5Ra / CD125 Protein

Synonym :	IL5Ra, Il5r
Protein Construction:	A DNA sequence encoding the mouse IL5Ra (P21183) (Met1-Val328) was expressed with a C-terminal polyhistidine tag.
Source:	Mouse
Expression Host:	Baculovirus-Insect cells

QC Testing von IL5Ra / CD125 Protein

Purity:	> 95 % as determined by SDS-PAGE	SDS-PAGE: IL5Ra protein
Endotoxin:	< 1.0 EU per μg of the protein as determined by the LAL method
Stability:	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal:	Asp 18
Molecular Mass:	The recombinant mouse IL5Ra comprises 321 amino acids and has a predicted molecular mass of 36.8 kDa. The apparent molecular mass of the protein is approximately 45 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation:	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von IL5Ra / CD125 Protein

Storage:	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Bescreibung von IL5Ra / CD125 Protein

Interleukin-5 receptor subunit alpha, also known as IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA and CD125, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and type 5 subfamily. IL5Ra / CD125 is expressed on eosinophils and basophils. IL5Ra / CD125 is the receptor for interleukin-5. The alpha chain binds to IL5. It is a heterodimer of an alpha and a beta subunit. The beta subunit is common to the IL3, IL5 and GM-CSF receptors. It interacts with SDCBP. The WSXWS motif of IL5Ra / CD125 appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif of IL5Ra / CD125 is required for JAK interaction and/or activation. The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4.

References

Murata Y. et al.,1992, J Exp Med. 175 (2): 341-51.
Ogata N. et al.,1998, Blood. 91 (7): 2264-71.
Laundy GJ. et al., 2003, Eur J Immunogenet. 30 (1):13-87.
Cen Osman. et al., 2003, J Biol Chem. 278 (10): 8837-45.
Namkung JH. et al., 2007, Allergy. 62 (8): 934-42.