ICOSLG / B7-H2 / ICOSL / CD275 Protein

16.05.2013

Sino Biological Inc. liefert ICOSLG / B7-H2 / ICOSL / CD275 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über ICOSLG / B7-H2 / ICOSL / CD275 Protein lesen Sie bitte: http://www.sinobiological.com/ICOS-Ligand-B7-H2-Protein-Antibody-a-615.html

Produkt-Information von ICOSLG / B7-H2 / ICOSL / CD275 Protein

Synonym	ICOSLG, B7H2, B7RP1, ICOSL, KIAA0653
Protein Construction	A DNA sequence encoding the human ICOSLG (O75144-1) (Met1-Ser258) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von ICOSLG / B7-H2 / ICOSL / CD275 Protein

Purity	(96.1+2.0)% as determined by SDS-PAGE	SDS-PAGE: ICOSLG protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Asp 19
Molecular Mass	The recombinant human ICOSLG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 481 amino acids and has a predicted molecular mass of 53.7 kDa. The apparent molecular mass of the protein is approximately 66-76 and 33 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von ICOSLG / B7-H2 / ICOSL / CD275 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ICOSLG / B7-H2 / ICOSL / CD275 Protein

Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. ICOSL constructs lacking either the IgC or IgV domain demonstrates that receptor binding is mediated solely by the IgV domain but requires the IgC domain for maintaining the structural integrity of the protein. The receptor binding site on ICOSL is constituted chiefly by aromatic / hydrophobic residues.

References

Wiendl H. et al., 2003, Brain. 126 (5): 1026-35.
Chattopadhyay K J. et al., 2006, Immunol. 177 (6): 3920-9.