SULT1A3 Protein (His Tag)

08.10.2012

Produkt-Information von SULT1A3 Protein

Synonym	SULT1A3, STM,SULT1A4
Protein Construction	A DNA sequence encoding the mature form of human SULT1A3 (P50224-1) (Glu2-Leu295) was expressed with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von SULT1A3 Protein

Purity	> 94 % as determined by SDS-PAGE	SDS-PAGE: SULT1A3 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human SULT1A3 consists of 301 amino acids and predicts a molecular mass of 30.5 KDa. It migrates as an approximately 34 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von SULT1A3 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SULT1A3 Protein

SULT1A3 is a member of the sulfotransferase 1 family. Sulfotransferase enzymes are different in their tissue distributions and substrate specificities while their gene structure (number and length of exons) is similar. They catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. SULT1A3 gene encodes a phenol sulfotransferase with thermolabile enzyme activity. It is the most centromeric of the four sulfotransferase genes. Exons of this gene overlap with exons of a gene that encodes a protein containing GIY-YIG domains (GIYD1). SULT1A3 can be detected in liver, colon, kidney, lung, brain, spleen, small intestine, placenta and leukocyte. SULT1A3 is a sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.

References

Dajani R. et al., 1999, Protein Expr Purif 16 (1): 11-8.
Dajani R. et al., 2000, J Biol Chem. 274 (53): 37862-8.
Yasuda S. et al., 2011, Toxicol Appl Pharmacol. 251 (2): 104-9.
Hildebrandt MA. et al., 2004, Biochem Biophys Res Commun. 321 (4): 870-8.
Lu JH. et al., 2005, Biochem Biophys Res Commun. 335 (2): 417-23.

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