WWP2 Protein (GST Tag)

08.10.2012

Produkt-Information von WWP2 Protein

Synonym	WWP2
Protein Construction	A DNA sequence encoding the human WWP2 (O00308) (Met1-Glu870) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von WWP2 Protein

Purity	> 90% as determined by SDS-PAGE	SDS-PAGE: WWP2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human WWP2 /GST chimera consists of 1107 amino acids and has a calculated molecular mass of 126.7 kDa. The recombinant protein migrates as an approximately 120 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 8.0, 10%glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von WWP2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von WWP2 Protein

WWP2 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It contains 1 C2 domain, 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain and 4 WW domains. WWP2 is highly expressed in undifferentiated embryonic stem cells and expression is reduced after embryoid body (EB) formation. It can be detected in heart, throughout the brain, placenta, lung, liver, muscle, kidney and pancreas. It is also expressed in spleen and peripheral blood leukocytes. WWP2 interacts with POU5F1, RBP1, EGR2, SLC11A2, SCNN1A, SCNN1B, SCNN1G, WBP1, WBP2, ATN1, adenovirus type 2 PIII, ERBB4, NDFIP1 and NDFIP2. WWP2 polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation. It also ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. SLC11A2 and RPB1 can also be ubiquitinated by WWP2.

References

McDonald FJ. et al., 2002, Am J Physiol Renal Physiol. 283 (3): F431-6.
Soond SM. et al., 2011, Oncogene. 30 (21): 2451-62.
Marcucci R. et al., 2011, EMBO J. 30 (20): 4211-22.
Maddika S. et al., 2011, Nat Cell Biol. 13 (6): 728-33.
Xu H. et al., 2009, Cell Res. 19 (5): 561-73.
Rougier JS. et al., 2005, Am J Physiol Cell Physiol. 288 (3): C692-701.
Galinier R. et al., 2002,Biochemistry. 41 (48): 14299-305.

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