SUB1 Protein (His Tag)

26.03.2013

Sino Biological Inc. liefert SUB1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über SUB1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/SUB1-PC4-Protein-Antibody-a-5740.html

Produkt-Information von SUB1 Protein

Synonym	SUB1, PC4, RPO2TC1
Protein Construction	A DNA sequence encoding the mature form of human SUB1 (P53999) (Met1-Leu127) was expressed with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von SUB1 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: SUB1 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human SUB1 consists of 142 amino acids and predicts a molecular mass of 16.2 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von SUB1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SUB1 Protein

SUB1 is a member of the transcriptional coactivator PC4 family. SUB1 binds single-stranded DNA. Single-stranded DNA-binding proteins play many roles in nucleic acid metabolism, but their importance during transcription remains unclear. SUB1 exhibits strong genetic interactions with factors necessary for promoter melting. It localizes near the transcription bubble in vitro and binds to promoters in vivo dependent upon preinitiation complexes assembly. It is a general coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. SUB1 interacts with the nontemplate strand of RNApII complexes during initiation. It may also be involved in stabilizing the multiprotein transcription complex.

References

Knaus R. et al., 1996, EMBO J. 15 (8): 1933-40.
Ge H. et al., 1994, Cell. 78 (3): 513-23.
Kaiser K. et al., 1994, Cell. 78 (3): 525-34.