APAP-1 / AAP1 / RYBP Protein (GST Tag)

26.03.2013

Sino Biological Inc. liefert APAP-1 / AAP1 / RYBP Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über APAP-1 / AAP1 / RYBP Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/RYBP-Protein-Antibody-a-5744.html

Produkt-Information von APAP-1 / AAP1 / RYBP Protein

Synonym	RYBP, DEDAF, YEAF1
Protein Construction	A DNA sequence encoding the human RYBP (Q8N488) (Met1-Phe228) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von APAP-1 / AAP1 / RYBP Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: RYBP protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human RYBP /GST chimera consists of 465 amino acids and has a calculated molecular mass of 52.6 kDa. The recombinant protein migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol, 0.5mM PMSF. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von APAP-1 / AAP1 / RYBP Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von APAP-1 / AAP1 / RYBP Protein

APAP-1, also known as AAP1 and RYBP, is widely expressed. It is highest expressed in lymphoid tissues and placenta. It may bind to DNA. APAP-1 contains 1 RanBP2-type zinc finger. APAP-1 inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. APAP-1 also promotes apoptosis. It may be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1.

References

Li Mao. et al., 2009, EMBO Rep. 10 (2): 166-72.
Schlisio. et al., 2002, EMBO J. 21 (21): 5775-86.
Peter M E. et al., 2001, J Biol Chem. 276 (34): 31945-52.