STX8 / Syntaxin 8 Protein (His Tag)

09.06.2013

Sino Biological Inc. liefert STX8 / Syntaxin 8 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über STX8 / Syntaxin 8 Protein (His Tag) lesen Sie bitte:http://www.sinobiological.com/STX8-Syntaxin-8-Protein-Antibody-a-6257.html

Produkt-Information von STX8 / Syntaxin 8 Protein

Synonym	STX8
Protein Construction	A DNA sequence encoding the human STX8 (Q9UNK0) (Met1-Gly215) was expressed with an N-terminal polyhistidine tag.
Source	Human
Expression Host	Human Cells

QC Testing von STX8 / Syntaxin 8 Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: STX8 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human STX8 comprises 235 amino acids and has a predicted molecular mass of 27 kDa. The apparent molecular mass of the protein is approximately 34 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von STX8 / Syntaxin 8 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von STX8 / Syntaxin 8 Protein

STX8, also known as syntaxin 8, directly interacts with HECTd3. STX8 forms the SNARE complex with syntaxin 7, vti1b and endobrevin. STX8 belongs to the syntaxin family. Members of this family are key molecules implicated in diverse vesicle docking and membrane fusion events. STX8 physically interacts with cystic fibrosis transmembrane conductance regulator (CFTR): recombinant syntaxin 8 binds CFTR in vitro and both proteins co-immunoprecipitate in HT29 cells. Syntaxin 8 regulates CFTR-mediated currents in chinese hamster ovary (CHO) cells stably expressing CFTR and syntaxin 8. STX8 contributes to the regulation of CFTR trafficking and chloride channel activity by the SNARE machinery.

References

Steegmaier M. et al., 1999, J Biol Chem. 273 (51): 34171-9.
Thoreau V. et al., 1999, Biochem Biophys Res Commun. 257 (2): 577-83.
Zhang L. et al., 2009, Cell Mol Neurobiol. 29 (1): 115-21.
Bilan F. et al., 2004, J Cell Sci. 117 (10): 1923-35.