SRI / Sorcin Protein (GST Tag)

10.01.2013

Sino Biological Inc. liefert SRI / Sorcin Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über SRI / Sorcin Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/SRI-Sorcin-Protein-g-10324.html

Produkt-Information von SRI / Sorcin Protein

Synonym	SRI
Protein Construction	A DNA sequence encoding the human SRI (P30626) (Met1-Val198) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von SRI / Sorcin Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: SRI protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human SRI /GST chimera consists of 435 amino acids and has a calculated molecular mass of 49.5 kDa. The recombinant protein migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von SRI / Sorcin Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SRI / Sorcin Protein

Sorcin is a alcium-binding protein which was originally identified in multidrug-resistant cells. It has been shown that sorcin serves as a modulator of the ryanodine receptor intracellular calcium channel and to modulate intracellular calcium cycling and calcium influx pathways in the heart. Sorcin may play an important role in the emergence of multidrug resistance in leukemia cells via regulating cell apoptosis pathways. It is over-expressed in multidrug-resistant leukemia cells. Over-expression of sorcin protein in K562 cells leads to significantly reduced cytosolic calcium level and increases resistance to cell death by apoptosis.

References

Meyers MB. et al., 1995, J Biol Chem. 270 (44): 26411-8.
Brownawell AM. et al., 1997, J Biol Chem. 272 (35): 22182-90.
Hansen. et al., 2003, FEBS Lett. 545 (2-3): 151-4.