COL4A3BP Protein (GST Tag)

10.01.2013

Sino Biological Inc. liefert COL4A3BP Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über COL4A3BP Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/COL4A3BP-Protein-g-10323.html

Produkt-Information von COL4A3BP Protein

Synonym	COL4A3BP, CERT, STARD11
Protein Construction	A DNA sequence encoding the human COL4A3BP (Q9Y5P4-1) (Met1-Phe598) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

COL4A3BP Protein QC Testing

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: COL4A3BP protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human COL4A3BP/GST chimera consists of 835 amino acids and has a calculated molecular mass of 95.8 kDa. The recombinant protein migrates approximately 96 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von COL4A3BP Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von COL4A3BP Protein

COL4A3BP is a member of the StarD2 subfamily. It contains a pleckstrin homology domain at its amino terminus and a START domain towards the end of the molecule. COL4A3BP has a lipid-binding domain that mediates intracellular trafficking of ceramide in a non-vesicular manner. One isoform of COL4A3BP is also involved in ceramide intracellular transport. COL4A3BP specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. An autoimmune response directed at this antigen can cause goodpasture disease.

References

Rual JF. et al., 2005, Nature. 437 (7062): 1173-8.
Granero F. et al., 2005, FEBS J. 272 (20): 5291-305.
Longo I. et al., 2006, Nephrol Dial Transplant. 21 (3): 665-71.