ATG8 / GABARAPL1 Protein (His Tag)

10.01.2013

Sino Biological Inc. liefert ATG8 / GABARAPL1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über ATG8 / GABARAPL1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/ATG8-GABARAPL1-Protein-g-10325.html

Produkt-Information von ATG8 / GABARAPL1 Protein

Synonym	GABARAPL1,GEC1
Protein Construction	A DNA sequence encoding the mature form of human GABARAPL1 (Q9H0R8) (Met1-Val225) was expressed with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von ATG8 / GABARAPL1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: ATG8 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human GABARAPL1 consists of 132 amino acids and predicts a molecular mass of 15.9 KDa. It migrates as an approximately 20-22 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, PH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von ATG8 / GABARAPL1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ATG8 / GABARAPL1 Protein

ATG8, also known as GABARAPL1, is a ubiquitin-like protein which consists of a 5-stranded β-sheet. ATG8 plays a key role in autophagy, the cellular process mediating the lysosome/vacuole-dependent turnover of macromolecules and organelles. ATG8 is especially important in macroautophagy. It is localized in the cytoplasm and at the PAS under nutrient-rich conditions, but becomes membrane-associated in case of autophagy induction. ATG8 then localizes to the site of autophagosome nucleation, the phagophore-assembly site. The subsequent recruitment of ATG8 and other autophagy-related proteins is believed to trigger vesicle expansion in a concerted manner, presumably by providing the driving force for membrane curvature. ATG8 is also required for a different autophagy-related process called the Cytoplasm-to-vacuole targeting (Cvt) pathway.

References

Ohsumi Y. et al., 2004, Genes Cells. 9 (7): 611-8.
Geng J. et al., 2008, EMBO Rep. 9 (9): 859-644.
Suzuki NN. et al., 2005, Autophagy. 1 (2): 119-26.