SIRPA / CD172A Antikörper ( FITC )

Produkt-Information von SIRPA / CD172A Antikörper

Usage Guide von SIRPA / CD172A Antikörper

Specificity	Human SIRPA / CD172A
	No cross-reactivity in WB and ELISA with Human cell lysate (293 cell line)
Flow Cytometry	Cells should be Fc-blocked by treatment with 20 μg of human IgG/106 cells for 1 hour at 4 ℃ prior to staining, washed, then stained with FITC Mouse anti-SIRPα (CD172a). Profile of anti-SIRPα (CD172a) reactivity on U937 cells analyzed by flow cytometry. Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human SIRPA in WB. Using a DAB detection system, the detection limit for Human SIRPA is approximately 1 ng/lane under non-reducing conditions and reducing conditions.
Storage	This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Verwandte Produkte & Themen von SIRPA / CD172A Antikörper

Immunology>>Adhesion Molecule>>Cell Adhesion Molecule (IgSF CAM)>>SIRP alpha/CD172a

Immunology>>Cluster of Differentiation>>SIRP alpha/CD172a

Hintergrund von SIRPA / CD172A Antikörper

Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains twoIg-like C1-type (immunoglobulin-like) domains and oneIg-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

References

1. Timms J.F. et al., 1999, Curr. Biol. 9: 927-30.
2. Stofega M.R. et al., 2000, J. Biol. Chem. 275: 28222-9.
3. Liu T. et al., 2005, J. Proteome Res. 4:2070-80.
4. Wolf-Yadlin A. et al., 2007, Proc. Natl. Acad. Sci.USA.104: 5860-5.

Sino Biological Inc. liefert SIRPA / CD172A Antikörper ( FITC ) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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