SELP / selectin P / P-selectin Protein (His Tag)

28.03.2012

Produkt-Information von SELP / selectin P / P-selectin Protein

Synonym	RP1-86F14.2, CD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL
Protein Construction	A DNA sequence encoding the human SELP (AAN06828.1) extracellular domain (Met 1- Ala 771) was fused with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

SELP / selectin P / P-selectin Protein QC Testing

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: SELP / selectin P / P-selectin protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Trp 42
Molecular Mass	The secreted recombinant human SELP consists of 741 amino acids and has a predicted molecular mass of 81.3 kDa. The apparent molecular mass of the protein is approximately 120 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH7.4 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

SELP / selectin P / P-selectin Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SELP / selectin P / P-selectin Protein

P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.

References

Johnson-Tidey RR. et al., 1994, Am J Pathol. 144 (5): 952-61.
Walcheck B. et al., 1996, J Clin Invest. 98 (5): 1081-7.
Foreman KE. et al., 1994, J Clin Invest. 94 (3): 1147-55.

Sino Biological Inc. liefert SELP / selectin P / P-selectin Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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