RUVBL1 / RVB1 Protein (His Tag)

09.01.2013

Sino Biological Inc. liefert RUVBL1 / RVB1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über RUVBL1 / RVB1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/RUVBL1-RVB1-Protein-g-10319.html

Produkt-Information von RUVBL1 / RVB1 Protein

Synonym	RUVBL1, INO80H, NMP238, TIP49, TIP49A
Protein Construction	A DNA sequence encoding the human RUVBL1(Q9Y265-1)(Met1-Lys456) was fused with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von RUVBL1 / RVB1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: RUVBL1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human RUVBL1 consists of 474 amino acids and has a calculated molecular mass of 52.4 kDa. The recombinant protein migrates as an approximately 57 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von RUVBL1 / RVB1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreiung von RUVBL1 / RVB1 Protein

RUVBL1, also known as RVB1, is a member of the RuvB family. It also is a component of the NuA4 histone acetyltransferase complex. RUVBL1 is ubiquitously expressed with high expression in heart, skeletal muscle and testis. It possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. It also is essential for cell proliferation. RUVBL1 may be able to bind plasminogen at cell surface and enhance plasminogen activation. RUVBL1 is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A.

References

Bauer A. et al., 1998, Proc Natl Acad Sci. 95 (25): 14787-92.
Ewing. et al., 2007, Mol Syst Biol. 3 (1): 89.
Puri T. et al., 2007, J Mol Biol. 366 (1): 179-92.