IVD / Isovaleryl-CoA dehydrogenase Protein (His Tag)

09.01.2013

Sino Biological Inc. liefert IVD / Isovaleryl-CoA dehydrogenase Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über IVD / Isovaleryl-CoA dehydrogenase Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/IVD-Isovaleryl-CoA-dehydrogenase-Protein-g-10320.html

Produkt-Information von IVD / Isovaleryl-CoA dehydrogenase Protein

Synonym	IVD
Protein Construction	A DNA sequence encoding the human IVD (AAH17202.1)(His30-His423) was fused with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von IVD / Isovaleryl-CoA dehydrogenase Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: IVD protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human IVD consists of 412 amino acids and has a calculated molecular mass of 45.3 kDa. The recombinant protein migrates as an approximately 43 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM Tris, 100mM Nacl, pH 8.0, 10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von IVD / Isovaleryl-CoA dehydrogenase Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von IVD / Isovaleryl-CoA dehydrogenase Protein

Isovaleryl-CoA dehydrogenase, also known as IVD, helps process a particular amino acid called leucine. Specifically, isovaleryl-CoA dehydrogenase is responsible for the third step in the breakdown of leucine. This step is a chemical reaction that converts a molecule called isovaleryl-CoA to another molecule, 3-methylcrotonyl-CoA. Additional chemical reactions convert 3-methylcrotonyl-CoA into molecules that are used for energy. The body breaks down proteins from food into smaller parts called amino acids. Amino acids can be further processed to provide energy for growth and development. In cells throughout the body, isovaleryl-CoA dehydrogenase exists in mitochondria. Mitochondria convert energy from food to a form that cells can use.

References

BACHHAWAT BK. et al., 1956, J Biol Chem. 219 (2): 539-50.
Ikeda Y. et al., 1983, J Biol Chem. 258 (2): 1077-85.
Tanaka K. et al., 1966, J Biol Chem. 219 (2): 539-50.