B3GNT6 (aa 44-384) Protein (His Tag)

09.01.2013

Sino Biological Inc. liefert B3GNT6 (aa 44-384) Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über B3GNT6 (aa 44-384) Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/B3GNT6-Protein-g-10318.html

Produkt-Information von B3GNT6 Protein

Synonym	B3GNT6
Protein Construction	A DNA sequence encoding the human B3GNT6 (Gln44-Ser384) was fused with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von B3GNT6 Protein

Purity	> 90% as determined by SDS-PAGE	SDS-PAGE: B3GNT6 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human B3GNT6 consists of 357 amino acids and has a calculated molecular mass of 40 kDa. The recombinant protein migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von B3GNT6 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von B3GNT6 Protein

B3GNT6 is a member of the glycosyltransferase 31 family. B3GNT6 play s an important role in the synthesis of mucin-type O-glycans. It catalyzes the transfer of GlcNAc from UDP-GlcNAc to GalNAcalpha1-Ser/Thr (Tn antigen) to form the core 3 structure (GlcNAcbeta1-3GalNAcalpha1-Ser/Thr) which exists in O-glycan. O-glycan is an important precursor in the biosynthesis of mucin-type glycoproteins. Loss of core 3 could lead to the production of secreted mucins, then bacteria would be inefficiently cleared from the system, and chronic inflammation would be developed, which eventually would result in development of cancer. B3GNT6 gene is a tumor suppressor gene.

References

Hennet T. et al., 1998, J Biol Chem. 273 (1): 58-65.
Kolbinger F. et al., 1998, J Biol Chem. 273 (1): 433-40.
Henrissat B. et al., 1997, Biochem J. 326: 929-39.