ROBO2 Protein (His Tag)

08.01.2013

Sino Biological Inc. liefert ROBO2 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über ROBO2 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/ROBO2-Protein-g-10310.html

Produkt-Information von ROBO2 Protein

Synonym	ROBO2, KIAA1568
Protein Construction	A DNA sequence encoding the human ROBO2 (Q9HCK4-1) (Met1-Pro859) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von ROBO2 Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: ROBO2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ser 22
Molecular Mass	The recombinant human ROBO2 consists of 849 amino acids and predicts a molecular mass of 94.2 KDa. It migrates as an approximately 116 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von ROBO2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ROBO2 Protein

ROBO2 is a member of the ROBO family. ROBOs are a group of highly conserved transmembrane glycoproteins that make up a small subgroup of the immunoglobulin (Ig) superfamily. They are best known for their roles as receptors for the Slit family of repellent axon guidance cues. In structure, they are characterized by five C2-type Ig-like repeats, three fibronectin type III domains, a transmembrane region, and an intracellular domain with three (ROBO3) or four (ROBO1, 2) CC (conserved cytoplasmic) motifs. ROBO2 abrogates SLIT-ROBO signaling in vitro. ROBO2 is a receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.

References

Brose K. et al., 1999, Cell. 96 (6): 795-806.
Brose K. et al., 1999, Neuron. 22 (3): 463-73.
Nagase T. et al., 2001, DNA Res. 7 (4): 273-81.