NKG2D / CD314 (aa 78-216) Protein (His Tag)

08.01.2013

Sino Biological Inc. liefert NKG2D / CD314 (aa 78-216) Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über NKG2D / CD314 (aa 78-216) Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/NKG2D-CD314-Protein-g-10311.html

Produkt-Information von NKG2D / CD314 Protein

Synonym	NKG2D, KLRK1
Protein Construction	A DNA sequence encoding the human NKG2D (Phe78-Val216) was fused with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von NKG2D / CD314 Protein

Purity	> 90% as determined by SDS-PAGE	SDS-PAGE: NKG2D protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human NKG2D consists of 155 amino acids and has a calculated molecular mass of 18.3 kDa. The recombinant protein migrates as an approximately 22 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris,500mM NaCl, pH 8.0, 10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von NKG2D / CD314 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NKG2D / CD314 Protein

NKG2D, also known as CD314, consists of two disulphide-linked type II transmembrane proteins with short intracellular proteins uncapable to transduce signals. In order to transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D is an immune receptor. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control.

References

Houchins J. et al., 1991, J Exp Med. 173: 1017-102.
Bauer S. et al., 1999, Science. 285 (5428): 727-9.
Zafirova B. et al., 2011, Cell Mol Life Sci. 68 (21): 3519-29.