RhoA Antikörper

Sino Biological Inc. liefert RhoA Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über RhoA Antikörper lesen Sie bitte: http://www.sinobiological.com/RhoA-Antibody-g-10060.html

Produkt-Information von RhoA Antikörper

Usage Guide von RhoA Antikörper

Specificity	Human RhoA
Flow Cytometry	Flow cytometric analysis of Human RHOA expression in HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-RHOA (12441-MM03), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Direct ELISA	This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human RHOA.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hinergrund von RhoA Antikörper

Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein which belongs to thesmall GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila)). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.

References

1. Kiss C, et al.,1997, Cytogenet. Cell Genet. 79 (3-4): 228-30.
2. Anastasiadis,P.Z. et al., 2000, Nat Cell Biol. 2 (9): 637-44.
3. Yiu G, et al.,2006,Nat. Rev. Neurosci. 7 (8): 617-27.
4. Wang,HR. et al., 2006, Methods Enzymol. 406: 437-47.
5. Heo,J. et al.,2006, Biochemistry. 45 (48): 14481-9.

Primary Antibody Products