Rat SIRPA / SIRP alpha / CD172a Protein (His Tag)

24.06.2013

Sino Biological Inc. liefert Rat SIRPA / SIRP alpha / CD172a Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat SIRPA / SIRP alpha / CD172a Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/SIRP-alpha-CD172a-SIRPA-Protein-Antibody-a-1293.html

Produkt-Information von SIRPA / SIRP alpha / CD172a Protein

Synonym	SIRPA, Bit, Mfr, Ptpns1, Shps1, Sirp
Protein Construction	A DNA sequence encoding the rat SIRPA (P97710) (Met1-Asn373) was expressed with a polyhistidine tag at the C-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von SIRPA / SIRP alpha / CD172a Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: SIRPA protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Lys 32
Molecular Mass	The recombinant rat SIRPA comprises 353 amino acids and predicts a molecular mass of 39 kDa. The apparent molecular mass of the recombinant protein is approximately 63-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von SIRPA / SIRP alpha / CD172a Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SIRPA / SIRP alpha / CD172a Protein

Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

References

Timms JF. et al., 1999, Curr Biol. 9: 927-30.
Stofega MR. et al., 2000, J Biol Chem. 275: 28222-9.
Liu T. et al., 2005, J Proteome Res. 4: 2070-80.
Wolf-Yadlin A. et al., 2007, Proc Natl Acad Sci. 104: 5860-5.