Rat CD150 / SLAM / SLAMF1 Protein (His Tag)

24.06.2013

Sino Biological Inc. liefert Rat CD150 / SLAM / SLAMF1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat CD150 / SLAM / SLAMF1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/CD150-SLAM-Protein-Antibody-a-328.html

Produkt-Information von CD150 / SLAM / SLAMF1 Protein

Synonym	SLAMF1
Protein Construction	A DNA sequence encoding the rat SLAMF1 (D3ZAD7) (Met1-Leu242) was expressed with a polyhistidine tag at the C-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von CD150 / SLAM / SLAMF1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: CD150 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Thr 25
Molecular Mass	The recombinant rat SLAMF1 comprises 229 amino acids and predicts a molecular mass of 26 kDa. The apparent molecular mass of the recombinant protein is approximately 44-47 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CD150 / SLAM / SLAMF1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CD150 / SLAM / SLAMF1 Protein

CD150/signaling lymphocytic activation molecule (SLAM) is a cell surface sialylated phosphoglycoprotein and belongs to the CD2 subset of the Ig superfamily of type I transmembrane glycoproteins. It has a 77-residue cytoplasmic tail containing several tyrosines within motifs for Src-homology 2 (SH2)-domain binding sites, and interacts with SH2 domain protein 1A (SH2D1A) and with PTPN11. LAM is constitutively expressed on peripheral-blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. As a self-ligand, CD150 performs diverse immunologic functions including T/B-cell costimulation, induction of interferon γ (IFN-γ) in Th1 T-cell clones, redirection of Th2 clones to a Th1 or Th0 phenotype, and inhibition of apoptosis in B cells. Furthermore, CD150 was shown to be the second receptor for measles virus in addition to CD46, and the distribution of SLAM on various cell lines is consistent with their susceptibility to clinical isolates of measles virus.

References

Svitlana V. et al., 1999, The Journal of Immunology. 162: 5719-27.
Duncan Howie. et al., 2002, Blood. 99 (3): 957-65.
Benjamin G. et al., 1995, Nature. 376: 260-3.
Hironobu Tatsuo. et al., 2000, Nature. 406: 893-7.
Svetlana P. et al., 2003,Nature Immunology. 4: 19-24.
Grant Welstead. et al., 2004, Journal of Virology. 78 (18): 9666-74.