Hamster FUT8 (aa 68-575) Protein (His Tag)

24.06.2013

Sino Biological Inc. liefert Hamster FUT8 (aa 68-575) Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Hamster FUT8 (aa 68-575) Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/FUT8-Protein-a-2597.html

Produkt-Information von FUT8 Protein

Synonym	FUT8
Protein Construction	A DNA sequence encoding the hamster FUT8 (G3HCE4) (Arg68-Lys575) was fused with a polyhistidine tag at the C-terminus.
Source	Hamster
Expression Host	Baculovirus-Insect cells

QC Testing von FUT8 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: FUT8 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Arg 575
Molecular Mass	The recombinant hamster FUT8 consists of 518 amino acids and has a calculated molecular mass of 60.1 kDa. The recombinant protein migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von FUT8 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von FUT8 Protein

Alpha (1,6) fucosyltransferase 8, also known as FUT8, is a member of the glycosyltransferase family. Fucosyltransferases are the enzymes transferring fucose from GDP-Fuc to Gal in an alpha1,2-linkage and to GlcNAc in alpha1,3-linkage, alpha1,4-linkage, or alpha1,6-linkage. All fucosyltransferases utilize the same nucleotide sugar, their specificity reside in the recognition of the acceptor and in the type of linkage formed. Fucosyltransferases share some common structural and catalytic features. On the basis of protein sequence similarities, these enzymes can be classified into four distinct families: (1) the alpha-2-fucosyltransferases, (2) the alpha-3-fucosyltransferases, (3) the mammalian alpha-6-fucosyltransferases, and (4) the bacterial alpha-6-fucosyltransferases. The alpha-3-fucosyltransferases constitute a distinct family as they lack the consensus peptide, but some regions display similarities with the alpha-2 and alpha-6-fucosyltranferases.

References

Breton C. et al., 1998, Glycobiology. 8 (1): 87-94.
Oriol R. et al., 1999, Glycobiology. 9 (4): 323-34.
De Vries T. et al., 2001, Glycobiology. 11 (10): 119-28.
Baboval T. et al., 2002, Mamm Genome. 13: 538-41.