Rat MPL / MPLV Protein (His Tag)

16.04.2013

Sino Biological Inc. liefert Rat MPL / MPLV Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat MPL / MPLV Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/c-MPL-CD110-TPOR-Protein-Antibody-a-6057.html

Produkt-Information von MPL / MPLV Protein

Synonym	MPL
Protein Construction	A DNA sequence encoding the mature form of rat MPL (Met1-Ala500) was expressed with a polyhistide tag at the C-terminus.
Source	Rat
Expression Host	Baculovirus-Insect cells

QC Testing von MPL / MPLV Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: MPL protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gln 22
Molecular Mass	The recombinant rat MPL consists of 490 amino acids and predicts a molecular mass of 55.1 KDa. It migrates as an approximately 55 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10%glycerol, pH 7.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von MPL / MPLV Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von MPL / MPLV Protein

CD110, also known as c-MPL, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. It is expressed at a low level in a large number of cells of hematopoietic origin. C-MPL is homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. Thrombopoietin is the ligand for c-mpl. It was shown to be the major regulator of megakaryocytopoiesis and platelet formation. Defects in c-MPL are a cause of congenital amegakaryocytic thrombocytopeniawhich is a disease characterized by isolated thrombocytopenia and megakaryocytopenia with no physical anomalies. Defects in c-MPL also cause thrombocythemia type 2 and myelofibrosis with myeloid metaplasia.

References

Souyri M. et al., 1991, Cell. 63 (6): 1137-47.
Vigon I. et al., 1989, Hum Genet. 83 (2): 194-6.
Vigon I. et al., 1992, Proc Natl Acad Sci. 89 (12): 5640-4.
Kato T. et al., 1999, Stem Cells. 16 (5): 322-8.