Rat FLT1 / VEGFR1 Protein (His Tag)

08.03.2013

Sino Biological Inc. liefert Rat FLT1 / VEGFR1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat FLT1 / VEGFR1 Protein (His Tag) lesen Sie bitte:http://www.sinobiological.com/Frizzled-5-FZD5-Protein-g-10554.html

Produkt-Information von FLT1 / VEGFR1 Protein

Synonym	FLT1, Flt-1, Vegfr1
Protein Construction	A DNA sequence encoding the rat FLT1(P53767)(Met1-Glu758) was expressed with a polyhistidine tag at the C-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von FLT1 / VEGFR1 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: FLT1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Tyr 23
Molecular Mass	The recombinant rat FLT1 comprises 747 amino acids and predicts a molecular mass of 84 kDa. The apparent molecular mass of the recombinant protein is approximately 108-114 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von FLT1 / VEGFR1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FLT1 / VEGFR1 Protein Description

Vascular endothelial growth factor receptor 1, also known as VEGFR-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor and FLT1, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR-1 / FLT1 contains seven Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. VEGFR-1 / FLT1 is expressed mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. It is specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR-1 / FLT1 is not expressed in tumor cell lines. VEGFR-1 / FLT1 is an essential receptor tyrosine kinase that regulates mammalian vascular development and embryogenesis. EGF-induced angiogenesis requires inverse regulation of VEGFR-1 and VEGFR-2 in tumor-associated endothelial cells. VEGFR-1 / FLT1 is a receptor for VEGF, VEGFB and PGF. It has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.

References

Kendall RL. et al., 1993, Proc Natl Acad Sci. 90: 10705-9.
Starovasnik MA. et al., 1999, J Mol Biol. 293: 531-44.
Dutta D. et al., 2008, J Biol Chem. 283 (37): 25404-13.
Mittar S. et al., 2009, Exp Cell Res.315 (5): 877-89.