Rat CLEC4F Protein

27.04.2013

Sino Biological Inc. liefert Rat CLEC4F Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat CLEC4F Protein lesen Sie bitte: http://www.sinobiological.com/CLEC4F-CLECSF13-Protein-Antibody-a-5859.html

Produkt-Information von CLEC4F Protein

Synonym	CLEC4F, Clecsf13, Kclr
Protein Construction	A DNA sequence encoding the rat CLEC4F(NP_446205.1) (Arg70-Ser550) was expressed with two additional amino acids (Gly & Pro) at the N-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von CLEC4F Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE CLEC4F protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gly
Molecular Mass	The recombinant rat CLEC4F comprises 483 amino acids and predicts a molecular mass of 53.7 kDa. The apparent molecular mass of the recombinant protein is approximately 63-68 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CLEC4F Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CLEC4F Protein

CLEC4F, a member of C-type lectins, was firstly purified from rat liver extract with high binding affinity to fucose, galactose and N-acetylgalactosamine, and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated. Histochemical staining showed that mouse CLEC4F（mCLEC4F） is only expressed on F4/80+ cells localized in liver, and is undetectable in bone marrow, spleen, lymph nodes, or other tissues in adult mice. However, mCLEC4F is detected in the liver of embryonic day 11.5 (E11.5), which is 1.5 day earlier than the formation of liver (E10) and is 3.5 day earlier than the formation of bone marrow (E15-16). Moreover, recombinant mCLEC4F.Fc binds to alpha-galactoceramide in a Ca++-dependent manner, and both galactose and ceramide can partially inhibit CLEC4F.Fc binding to alpha-galactoceramide. Interestingly, mCLEC4F-deficient (mCEC4F k/o) mice produced far less cytokines than wild type littermates after intravenous injection ofalpha-galactoceramide. This suggests that mCLEC4F is not only a specific marker for Kupffer cells, but is also critical for the presentation of glycolipid antigen to NKT cells.

References

Shie-Liang Edmond Hsieh. et al., 2009, The Journal of Immunology. 182: 78.
Ota T. et al., 2004, Nat Genet. 36 (1): 40-5.
Bonaldo MF. et al., 1996, Genome Res. 6 (9): 791-806.

CLEC4F (CLECSF13) related areas, pathways, and other information