Rat CD276 / B7-H3 Protein (His Tag)

28.03.2013

Sino Biological Inc. liefert Rat CD276 / B7-H3 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat CD276 / B7-H3 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/B7-H3-CD276-Protein-a-1573.html

Produkt-Information von CD276 / B7-H3 Protein

Synonym	CD276, B7h3
Protein Construction	A DNA sequence encoding the rat CD276 (Q7TPB4)(Met1-Phe244) was expressed with a polyhistidine tag at the C-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von CD276 / B7-H3 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: B7-H3 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Val 29
Molecular Mass	The recombinant rat CD276 comprises 227 amino acids and predicts a molecular mass of 25 kDa. The apparent molecular mass of the recombinant protein is approximately 43 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CD276 / B7-H3 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CD276 / B7-H3 Protein

B7-H3 is one of the most recently identified members of the B7/CD28 superfamily of costimulatory molecules serving as an accessory modulator of T-cell response. B7 family molecules, which are expressed on antigen-presenting cells and display extracellular regions containing immunoglobulin (Ig) variable (V)- and constant (C)-like domains, are known to modulate T cell receptor (TCR)-mediated T cell activation by providing co-signals that are either stimulatory or inhibitory. B7-H3 provides a stimulatory signal to T cells. However, recent studies suggest a negative regulatory role for B7-H3 in T cell responses. B7-H3 inhibited T cell proliferation mediated by antibody to T cell receptor or allogeneic antigen-presenting cells. B7-H3 is a negative regulator that preferentially affects T(H)1 responses. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. Recently, B7-H3 expression has also been found in a variety of different human cancers, including prostate cancer, clear cell renal cell carcinoma (ccRCC), non-small-cell lung cancer (NSCLC), pancreatic cancer, gastric cancer, ovarian cancer, colorectal cancer (CRC) and urothelial cell carcinoma. B7-H3 was expressed in some human cancers and correlated with poor outcome of cancer patients.

References

Loos M. et al., 2010, Clin Dev Immunol. 2010: 683875.
Nagashima O. et al., 2008, J Immunol. 181 (6): 4062-71.
Suh WK. et al., 2003, Nat Immunol. 4 (9): 899-906.
Waschbisch A. et al., 2008, Arthritis Rheum. 58 (11): 3600-8.
Sun J. et al., 2010, Cancer Immunol Immunother. 59 (8): 1163-71.