Rat TrkA / NTRK1 Protein (His Tag)

28.03.2013

Sino Biological Inc. liefert Rat TrkA / NTRK1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat TrkA / NTRK1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/TrkA-NTRK1-Protein-Antibody-a-528.html

Produkt-Information von TrkA / NTRK1 Protein

Synonym	NTRK1, Trk, Trka
Protein Construction	A DNA sequence encoding the rat NTRK1 (Met1-Pro418) was expressed with a polyhistidine tag at the C-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von TrkA / NTRK1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: TrkA protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ala 33
Molecular Mass	The recombinant rat NTRK1 comprises 397 amino acids and predicts a molecular mass of 43.7 kDa. The apparent molecular mass of the recombinant protein is approximately 67-77 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von TrkA / NTRK1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von TrkA / NTRK1 Protein

Neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), also well known as TrkA, is a member of the neurotrophic tyrosine kinase receptor family belonging to Tyr protein kinase superfamily, which also includes TrkB and TrkC. TrkA/NTRK1 has a complex subdomain orgnazation conserved in the family members, containing two cysteine-rich domains, a cluster of three leucine-rich motifs (LRR), and two Ig-like C2-type domains in the extracellular region, as well as an tyrosine kinase domain in the cytoplasmic region. TrkA/NTRK1 exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures, and is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA/NTRK1 plays an important role in cell survival, differentiation, and apoptosis in various neuronal and nonneuronal cell types, especially in the nervous system by recruiting signaling molecules including SHC1, PI 3-kinase, and PLC-gamma-1 that activate pathways required for the growth and survival of neurons. It has been shown that TrkA/NTRK1 is specially involved in the development and function of the nociceptive reception system and establishment of thermal regulation via sweating. Two distinct TrkA/NTRK1 isoforms have been identified: TrkA-II is primarily expressed in neuronal cells, while isoform TrkA-I is found in non-neuronal tissues. Defects in TrkA/NTRK1 are a cause of a rare autosomal recessive disorder, congenital insensitivity to pain with anhidrosis (CIPA).

References

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Holtzman DM. et al., 2009 , J Neurosci. 15: 1567-76.
Dan C Lin. et al., 2006,Mol Cell Biol. 26: 8928-41.
Jung EJ. et al., 2008, Exp Mol Med. 40: 276-85.