Cynomolgus B7-H1 / CD274 / PD-L1 Protein (His Tag)

24.06.2013

Sino Biological Inc. liefert Cynomolgus B7-H1 / CD274 / PD-L1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus B7-H1 / CD274 / PD-L1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/PD-L1-B7-H1-CD274-Protein-Antibody-a-382.html

Produkt-Information von B7-H1 / CD274 / PD-L1 Protein

Synonym	CD274
Protein Construction	A DNA sequence encoding the cynomolgus CD274 (F6VEW6) (Met1-Thr239) was expressed with a polyhistidine tag at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von B7-H1 / CD274 / PD-L1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: B7-H1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Phe 19
Molecular Mass	The recombinant cynomolgus CD274 comprises 232 amino acids and has a calculated molecular mass of 26.7 KDa. The apparent molecular mass of it is approximately 37 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von B7-H1 / CD274 / PD-L1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von B7-H1 / CD274 / PD-L1 Protein

B7-H1, also known as CD274 and PD-L1, is a cell surface glycoprotein belonging to the B7 family of costimulatory molecules. B7-H1 is originally identified by homology analysis in comparison with B7-1 and B7-2, two molecules with important immunoregulatory functions. B7-H1 costimulates T-cell proliferation and preferentially induces IL-10 and IFN-gamma secretion in a PDCD1-independent manner. The costimulatory function of B7-H1 is critical for enhancing maturation and differentiation of T-cells in lymphoid organs. On contrast, B7-H1 also functions as a co-inhibitor through programmed death-1 (PD-1). By binding to PD-1 receptors on activated T-cells and B-cells, B7-H1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production, and thus inhibits ongoing T-cell responses in peripheral tissues. Aberrantly expressed of B7-H1 by tumor cells or lymphocytes is associated with aggressive pathologic features of certain carcinomas and represents a promising target for immune manipulation. Mouse and human B7-H1/PD-L1 share 73% amino acid sequence identity.

References

Petrella J. et al., 2002, J Virol. 76 (20): 10503-6.
Tamura H. et al., 2003, Int J Hematol. 78 (4): 321-8.
Holets L M. et al., 2006, Biol Reprod. 74 (2):352-8.
Thompson R H. et al., 2006, Clin Genitourin Cancer. 5 (3): 206-11.