Rat CD10 / MME / Neprilysin Protein (His Tag)

20.05.2013

Sino Biological Inc. liefert Rat CD10 / MME / Neprilysin Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat CD10 / MME / Neprilysin Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/CD10-Neprilysin-Protein-a-298.html

Produkt-Information von CD10 / MME / Neprilysin Protein

Synonym	MME
Protein Construction	A DNA sequence encoding the rat MME (P07861) (Tyr52-Trp750) was expressed with a polyhistidine tag at the N-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von CD10 / MME / Neprilysin Protein

Purity	>95 % as determined by SDS-PAGE	SDS-PAGE: CD10 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant rat MME comprises 719 amino acids and has a predicted molecular mass of 82.5 kDa. The apparent molecular mass of the protein is approximately 93 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CD10 / MME / Neprilysin Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CD10 / MME / Neprilysin Protein

Neprilysin, also known as neutral endopeptidase ( NEP ) and MME, is a type II transmembrane glycoprotein normally expressed by a variety of tissues, including epithelial cells of the prostate, kidney, intestine, endometrium, adrenal glands, and lung. This zinc-dependent metalloprotease enzyme cleaves peptide bonds on the amino side of hydrophobic residues and inactivates a variety of physiologically active secreted peptides. Neprilysin is thought to be the rate-limiting degrading enzyme of amyloid β peptide (Aβ) whose abnormal misfolding and aggregation in neural tissue has been implicated in the development of Alzheimer‘s disease (AD). MME is also identified as the common acute lymphoblastic leukemia antigen ( CALLA ) or CD10 present on leukemic cells of pre-B phenotype, and thus serves as the most important biomarker in the diagnosis of human acute lymphocytic leukemia (ALL). In addition, associations have been observed between neprilysin expression and various types of cancer, and in some cancer models NEP is demonstrated to negatively regulate angiogenesis through proteolytically inactivating the fibroblast growth factor-2 ( FGF-2 ). Accordingly, Loss or a decrease in neprilysin expression has been reported in a several malignancies, as well as Alzheimer's-like behavioral impairment and amyloid-beta deposition in the brain.

References

Shipp MA. et al. 1989, Proc Natl Acad Sci. 86: 297-301.
Goodman OB. et al., 2006, J Biol Chem. 281: 33597-605.
Cohen AJ. 1996, Cancer Res. 56: 831-9.
Iwata N. et al., 2001, Science. 292: 1550-2.
EI-Amouri SS. et al., 2008, Am J Pathol. 172: 1342-54.