Mouse M-CSF / CSF-1 Protein (His Tag)

20.05.2013

Sino Biological Inc. liefert Mouse M-CSF / CSF-1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse M-CSF / CSF-1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/M-CSF-CSF-1-Protein-g-11726.html

Produkt-Information von M-CSF / CSF-1 Protein

Synonym :	CSF1, Csfm
Protein Construction:	A DNA sequence encoding the mouse CSF1 (P07141-1) (Met1-Glu262) was expressed with a C-terminal polyhistidine tag.
Source:	Mouse
Expression Host:	Human Cells

QC Testing von M-CSF / CSF-1 Protein

Purity:	> 95 % as determined by SDS-PAGE	SDS-PAGE: M-CSF protein
Endotoxin:	< 1.0 EU per μg of the protein as determined by the LAL method
Stability:	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal:	Lys 33
Molecular Mass:	The recombinant mouse CSF1 comprises 241 amino acids and has a predicted molecular mass of 27.4 kDa. The apparent molecular mass of the protein is approximately 40-55 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation:	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von M-CSF / CSF-1 Protein

Storage:	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von M-CSF / CSF-1 Protein

Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

References

Pandit J. et al., 1992, Science. 258: 1358-62.
Tokai M. et al., 2000, J Bacteriol. 182 (10): 2865-8.
Fan X. et al., 2001, Am J Physiol Endocrinol Metab. 280 (1): E103-11.
Frangogiannis NG. et al., 2003, Am J Physiol Heart Circ Physiol. 285 (2): H483-92.
Cupp JS. et al., 2007, Am J Surg Pathol. 31 (6): 970-6.