Cynomolgus SIRPG Protein (His Tag)

21.05.2013

Sino Biological Inc. liefert Cynomolgus SIRPG Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus SIRPG Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/SIRPG-SIRP-gamma-CD172g-Protein-a-1482.html

Produkt-Informaiton von SIRPG Protein

Synonym	SIRPG
Protein Construction	A DNA sequence encoding the cynomolgus SIRPG (Met1-His360) was expressed with a polyhistidine tag at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von SIRPG Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: SIRPG protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu 29
Molecular Mass	The recombinant heterodimer of cynomolgus SIRPG comprises 343amino acids and has a calculated molecular mass of 38.1 KDa. The apparent molecular mass of the protein is approximately 45 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von SIRPG Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SIRPG Protein

Signal-regulatory protein gamma, also known as SIRP-gamma, CD172 antigen-like family member B, Signal-regulatory protein beta-2, SIRP-beta-2, CD172g and SIRPG, is a single-pass type I membrane protein. SIRPG / CD172g is detected in liver, and at very low levels in brain, heart, lung, pancreas, kidney, placenta and skeletal muscle. It is expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. SIRPG / CD172g contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. It is mainly present in the paracortical T-cell area of lymph nodes, with only sparse positive cells in the mantle and in the germinal center of B-cell follicles. In the thymus, SIRPG / CD172g is primarily expressed in the medulla on mature T lymphocytes that have undergone thymic selection. SIRPG / CD172g is probable immunoglobulin-like cell surface receptor. On binding with CD47, it mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.

References

Ichigotani Y. et al., 2000, J Hum Genet. 45: 378-82.
Van den Berg TK. et al., 2005, J Immunol. 175 (12): 7788-9.
Piccio L. et al., 2005, Blood. 105: 2421-7.
Hatherley D. et al., 2008, Mol Cell. 31: 266-77.