Rat ADAM17 Protein (His Tag)

11.03.2013

Sino Biological Inc. liefert Rat ADAM17 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat ADAM17 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/ADAM17-Protein-g-10569.html

Produkt-Information von ADAM17 Protein

Synonym	ADAM17, Tace
Protein Construction	A DNA sequence encoding the rat ADAM17 (Q9Z1K9)(Met1-Asp563) was expressed with a polyhistidine tag at the C-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von ADAM17 Protein

Purity	(73.2+23.7) % as determined by SDS-PAGE	SDS-PAGE: ADAM17 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Pro 18
Molecular Mass	The recombinant rat ADAM17 comprises 557 amino acids and predicts a molecular mass of 63 kDa. The apparent molecular mass of the recombinant protein is approximately 57 and 20 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von ADAM17 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ADAM17 Protein

ADAM17 is a member of the ADAM protein family of disintegrins and metalloproteases. ADAM17 is ubiquitously expressed in the human colon, with increased activity in the colonic mucosa of patients with ulcerative colitis, a main form of inflammatory bowel disease. The expression of ADAM17 may be inhibited by ethanol. It is involved in the processing of tumor necrosis factor alpha (TNF-α) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. ADAM17 also plays a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules, receptors, ligands, and enzymes. ADAM17 may play a prominent role in the Notch signaling pathway, during the proteolytic release of the Notch intracellular domain (from the Notch1 receptor) that occurs following ligand binding.

References

Poghosyan. et al., 2002, J Biol Chem. 277 (7): 4999-5007.
Li Y. et al., 2006, Blood. 108 (7): 2275-9.
Peiretti. et al., 2003, J Cell Sci. 116 (10): 1949-57.