Rat FZD4 / Frizzled-4 Protein

11.03.2013

Sino Biological Inc. liefert Rat FZD4 / Frizzled-4 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat FZD4 / Frizzled-4 Protein lesen Sie bitte: http://www.sinobiological.com/FZD4-Frizzled-4-Protein-g-10571.html

Produkt-Information von FZD4 / Frizzled-4 Protein

Synonym	FZD4
Protein Construction	A DNA sequence encoding the rat FZD4 (Q9QZH0) (Met1-Glu181) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von FZD4 / Frizzled-4 Protein

Purity	(13.3+78.9+5.2 )% as determined by SDS-PAGE	SDS-PAGE: FZD4 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Phe 38
Molecular Mass	The recombinant rat FZD4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 385 amino acids and has a predicted molecular mass of 43.2 kDa. The apparent molecular mass of the protein is approximately 113, 58 and 36 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von FZD4 / Frizzled-4 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage.Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von FZD4 / Frizzled-4 Protein

FZD4, also known as CD344, belongs to the the frizzle family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. FZD4 is the receptor for Wnt proteins. In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin, promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. FZD4 plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin.

References

Omoto S. et al., 2004, Ophthalmic Genet. 25 (2): 81-90.
Toomes C. et al., 2004, Invest Ophthalmol Vis Sci. 45 (7): 2083-90.
Sagara N. et al., 2001, Biochem Biophys Res. Commun. 282 (3): 750-6.