Rat SLC3A2 Protein (His Tag)

11.03.2013

Sino Biological Inc. liefert Rat SLC3A2 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat SLC3A2 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/SLC3A2-Protein-g-10568.html

Produkt-Information von SLC3A2 Protein

Synonym	SLC3A2
Protein Construction	A DNA sequence encoding the rat SLC3A2 (Q794F9) (Ala100-Ala527) was expressed with a polyhistidine tag at the N-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von SLC3A2 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: SLC3A2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant rat SLC3A2 comprises 448 amino acids and has a predicted molecular mass of 49.7 kDa. The apparent molecular mass of the protein is approximately 58 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von SLC3A2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage.Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SLC3A2 Protein

4F2 cell-surface antigen heavy chain, also known as 4F2 heavy chain antigen, Lymphocyte activation antigen 4F2 large subunit, CD98, SLC3A2 and MDU1, is a single-pass type I I membrane protein which belongs to the SLC3A transporter family. SLC3A2 / MDU1 is expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta is significantly stronger at full-term than at the mid-trimester stage. SLC3A2 / MDU1 is expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. It is expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. SLC3A2 / MDU1 is also expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco2-BBE. SLC3A2 / MDU1 is required for the function of light chain amino-acid transporters. It involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. SLC3A2 / MDU1 is involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7, SLC3A2 / MDU1 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. SLC3A2 / MDU1 plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. It is required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, SLC3A2 / MDU1 is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta.

References

Torrents D. et al., 1998, J Biol Chem. 273: 32437-45.
Pfeiffer R. et al., 1999, EMBO J. 18: 49-57.
Broeer A. et al., 2000, Biochem J. 349: 787-95.
Yanagida O. et al., 2001, Biochim Biophys Acta. 1514: 291-302.
Broeer A. et al., 2001, Biochem J. 355: 725-31.
Fort J. et al., 2007, J Biol Chem. 282: 31444-52.
Mayya V. et al., 2009, Sci Signal. 2: RA46-RA46.