RAMP3 Protein

04.03.2013

Sino Biological Inc. liefert RAMP3 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über RAMP3 Protein lesen Sie bitte: http://www.sinobiological.com/RAMP3-Protein-g-10540.html

Produkt-Information von RAMP3 Protein

Synonym	RAMP3
Protein Construction	A DNA sequence encoding the human RAMP3 (Met1-Val118) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von RAMP3 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: RAMP3 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Arg 24
Molecular Mass	The recombinant human RAMP3 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 336 amino acids and has a predicted molecular mass of 37.8 kDa. The apparent molecular mass of the protein is approximately 28-31 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von RAMP3 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von RAMP3 Protein

RAMP3 is a member of the RAMP family. Members of this family are single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. They have a wide biological distribution; high concentrations are found in the brain, lung, liver, heart and spleen with lower expression levels present in the testes, gastrointestinal tract and thyroid. They are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of RAMP3 protein, CRLR functions as an adrenomedullin receptor.

References

Stelzl U. et al., 2005, Cell. 122 (6): 957-68.
Scherer SW. et al., 2003, Science. 300 (5620): 767-72.
Kuwasako K. et al., 2004, Mol Pharmacol. 65 (1): 207-13.