RACK1 / GNB2L1 Protein (MBP Tag)

17.04.2012

Produkt-Information von RACK1 / GNB2L1 Protein

Synonym	HLC7, Gnb2-rs1, H12.3, HLC-7, PIG21, RACK1
Protein Construction	A DNA sequence encoding the human GNB2L1 (P63244 ) (Met 1-Arg 317) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von RACK1 / GNB2L1 Protein

Purity	> 83 % as determined by SDS-PAGE	SDS-PAGE: RACK1 / GNB2L1 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human GNB2L1/MBP fusion protein consists of 714 amino acids and has a calculated molecular mass of 78.7 KDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH 7.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von RACK1 / GNB2L1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von RACK1 / GNB2L1 Protein

RACK1 (guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1), also known as GNB2L1, belongs to the WD repeat G protein beta family. RACK1 facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. It modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. RACK1 is involved in PKC-dependent translocation of ADAM12 to the cell membrane. GNB2L1 promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. It is required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. GNB2L1 also is required for PTK2 phosphorylation and dephosphorylation. It regulates internalization of the muscarinic receptor CHRM2. It also promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L.

References

Jannot G. et al., 2011, EMBO Rep. 12 (6): 581-6.
Wang F. et al., 2011, J Biol Chem. 286 (11): 9097-106.
Cao XX. et al., 2011, Breast Cancer Res Treat. 126 (3): 555-63.
Myklebust LM. et al., 2011, Thyroid. 21 (11): 1217-25.

Sino Biological Inc. liefert RACK1 / GNB2L1 Protein (MBP Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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