DEP1 / PTPRJ (aa 997-1337) Protein (His Tag)

17.04.2012

Produkt-Information von PTPRJ Protein

Synonym	CD148, DEP1, HPTPeta, R-PTP-ETA, SCC1
Protein Construction	A DNA sequence encoding the human PTPRJ (Q12913) cytoplasmic domain (Arg 997-Ala 1337) was expressed, with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von PTPRJ Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: PTPRJ protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human PTPRJ (aa 997-1337) consists of 352 amino acids and has a calculated molecular mass of 41KDa. It migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of PBS. pH 7.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von PTPRJ Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PTPRJ Protein

Receptor-type tyrosine-protein phosphatase eta (DEP1 / PTPRJ) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. DEP1 / PTPRJ possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. DEP1 / PTPRJ is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. In stable MCF-7 cell lines lines, induction of DEP-1 expression inhibited breast cancer cell growth by 5–10-fold. These data describe PTPs expressed and regulated in breast cancer cell lines during differentiation and identify one PTP, DEP-1, that inhibits the growth of breast cancer cells in vitro.

References

Holsinger LJ. et al., 2002, Oncogene. 21 (46): 7067-76.
Huang X. et al., 2009, Nat Genet. 41 (4): 494-7.
Kuramochi S. et al., 1996, FEBS Lett. 378 (1): 7-14.

Sino Biological Inc. liefert DEP1 / PTPRJ (aa 997-1337) Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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