CALML3 Protein (His Tag)

17.04.2012

Produkt-Information von CALML3 Protein

Synonym	CLP
Protein Construction	A DNA sequence encoding the human CALML3 (P27482) (Met 1-Lys 149) was expressed, with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von CALML3 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: CALML3 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human CALML3 consisting of 164 amino acids and has a calculated molecular mass of 18.7KDa. It migrates as an 16 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CALML3 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CALML3 Protein

Calmodulin-like protein 3 (CALML3) is similar to that of authentic calmodulin and may actually compete with calmodulin by binding, with different affinity, to cellular substrates. Calmodulin-like protein 3 (CALML3) is a tumor-sensitive protein specifically expressed in normal epithelial cells but downregulated in tumorigenesis. Downregulation of the protein is an early event in breast cancer development. One of the most pressing questions raised by the discovery of CLP/CALML3 is that of its potential targets. Although it is 85% identical to human calmodulin, the distinct properties of CLP suggest that it has specific targets or targets that only partially overlap with those of calmodulin. Fluorescence microscopy and Western blotting revealed that CLP expression results in up-regulation of its target protein, myosin-10. This up-regulation occurs at the protein level by stabilization of myosin-10. Thus, CLP functions by increasing the stability of myosin-10, leading to enhanced myosin-10 function and a subsequent increase in filopodial dynamics and cell migration.

References

Bennett RD. et al., 2007, J Biol Chem. 282 (5): 3205-12.
Perochon A. et al., 2011, Biochimie. 93 (12): 2048-53.
Chinpongpanich A. et al., 2011, Acta Biochim Biophys Sin (Shanghai). 43 (11): 867-76.

Sino Biological Inc. liefert CALML3 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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