PTPN12 (aa 1-355) Protein (GST Tag)

02.05.2013

Sino Biological Inc. liefert PTPN12 (aa 1-355) Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über PTPN12 (aa 1-355) Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/PTPN12-Protein-Antibody-a-5921.html

Produkt-Information von PTPN12 Protein

Synonym	PTPN12
Protein Construction	A DNA sequence encoding the human PTPN12 (AAA36529.1) (Met1-Gln355) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von PTPN12 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: PTPN12 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human PTPN12 chimera consists of 592 amino acids and has a calculated molecular mass of 69.4 kDa. The recombinant protein migrates approximately 64 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von PTPN12 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PTPN12 Protein

PTPN12 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN12 contains a C-terminal PEST motif, which serves as a protein–protein interaction domain, and may be related to protein intracellular half-life. PTPN12 was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. PTPN12 was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobilit.

References

Garton AJ. et al., 1997, Oncogene. 15 (8): 877-85.
Lin Yi. et al., 2003, Am J Physiol Heart Circ. 285 (2): H710-21.
Takekawa M. et al., 1994, FEBS Lett. 339 (3): 222-8.