CD28 / TP44 Protein

02.05.2013

Sino Biological Inc. liefert CD28 / TP44 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CD28 / TP44 Protein lesen Sie bitte: http://www.sinobiological.com/CD28-Protein-a-306.html

Produkt-Information von CD28 / TP44 Protein

Synonym	CD28
Protein Construction	A DNA sequence encoding the human CD28 (P10747-1) (Met 1-Pro 152) was expressed with six amino acids (LEVLFQ) at the C- terminus.
Source	Human
Expression Host	Human Cells

QC Testing von CD28 / TP44 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: CD28 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Asn 19
Molecular Mass	The recombinant human CD28 consists of 141 amino acids and predicts a molecular mass of 15.9 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CD28 / TP44 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CD28 / TP44 Protein

T-cell-specific surface glycoprotein CD28, also known as TP44 and CD28, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. CD28 / TP44 is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. CD28 / TP44 is expressed in T-cells and plasma cells, but not in less mature B-cells. CD28 / TP44 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. CD28 / TP44 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. CD28 / TP44 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

References

Linsley PS. et al., 1993, Annu Rev Immunol. 11 :191-212.
Edmead CE. et al., 1997, Int J Biochem Cell Biol. 29 (8-9):1053-7.
Woerly G. et al., 2002, J Leukoc Biol. 72 (4): 769-79.