ERN1 / IRE1 (aa 465-977) Protein

02.05.2013

Sino Biological Inc. liefert ERN1 / IRE1 (aa 465-977) Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über ERN1 / IRE1 (aa 465-977) Protein lesen Sie bitte: http://www.sinobiological.com/ERN1-Protein-a-2364.html

Produkt-Information von ERN1 / IRE1 Protein

Synonym	ERN1
Protein Construction	A DNA sequence encoding the human ERN1 (O75460-1) (Pro 465-Leu 977) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von ERN1 / IRE1 Protein

Purity	> 80 % as determined by SDS-PAGE	SDS-PAGE: ERN1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gly
Molecular Mass	The secreted recombinant human ERN1 consists of 515 amino acids and predicts a molecular mass of 58.3 KDa. The apparent molecular mass of the protein is approximately 65 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von ERN1 / IRE1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ERN1 / IRE1 Protein

Serine / threonine-protein kinase/endoribonuclease IRE1, also known as, endoplasmic reticulum-to-nucleus signaling 1, inositol-requiring protein 1, Ire1-alpha, ERN1 and IRE1, is a single-pass type I membrane protein which belongs to the protein kinase superfamily and Ser/Thr protein kinase family. ERN1 contains one KEN domain and one protein kinase domain. ERN1 is ubiquitously expressed. High levels of ERN1 is observed in pancreatic tissue. ERN1 binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1. ERN1 also interacts with TAOK3 and TRAF2. ERN1 senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain of ERN1 splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.The kinase domain of ERN1 is activated by trans-autophosphorylation. Kinase activity of ERN1 is required for activation of the endoribonuclease domain.

References

Trentmann SM. et al., 2000, Plant Mol Biol. 44 (1): 11-25.
Iwawaki T. et al., 2001, Nat Cell Biol. 3: 158-64.
Liu CY. et al., 2003, J Biol Chem. 278: 17680-7.
Oppermann FS. et al., 2009, Mol Cell Proteomics. 8: 1751-64.